InCyt Basic PM¢â

 

  
 

                                              

                                                  (click image to enlarge and get detailed specs)

  Complete Turnkey System

  The InCyt Basic Pm¢â Photometry System includes everything needed for calcium and other single excitation
  and ratiometric fluorophore experiments.
  The system includes: an inverted epi-fluorescence/phase contrast microscope, photon counting photometer
  assembly, computer controlled filter changer, Mercury-Xenon lamp, data acquisition & analysis computer
  workstation and data acquisition/analysis software.




  Ease-of-use

  The InCyt Basic Pm¢â Photometry System was designed in a biomedical research laboratory by scientists for
  scientists.
  Menu selection appears in the sequence in which experiments are performed.
  Esoteric options that add complexity and are rarely used have been eliminated from the menus.
  The software follows standard Windows graphical-user-interface-protocol.
  The hardware has been tested for reliability and ruggedness, as well as, simplicity of set up and operation.
  New users can learn to use the system and be doing their first experiment in a few hours.





  Data Collection, Analysis & Presentation

  Data can be measured continuously in user definable regions of interest.
  The results are displayed in real time and can be saved for post hoc analysis.
  Ion concentration within cells or ratio values can be displayed.
  Data files generated by InCyt Basic Pm¢â are stored as ASCII text.

 
 

                                                      Specifications

                                                                 Photometer Module
 
 PMT
 Hamamatsu HC135-11 photon counter
 Linearity(precision)
 +/-1% from 0 to 20,000,000
 counts/second
 Maximum
 Dark Count(noise)
 150 counts/second
 Equivalent
 Noise Input(noise)
 3 x 10-17 watts per second (measured
 at 400nm)
 Spectral
 Sensitivity(noise)
 300-650nm
 Discriminator  &
 Microprocessor
 Built into PMT
 Overload
 Protection
 Computer-controlled shutter (optional)
 Excitation
 120 watt Mercury-Xenon arc lamp for
 both UV and visible light excitation.
 Variable intensity
 Filter Changer
 Sutter Wheel Filter Changer

 
  Image Acquisition and Data Analysis Workstation
 
 CPU
 2.80 Ghz Pentium 4
 RAM
 512 MB
 Hard Drive
 80 GB
 CD ROM
 48x CD R/W
 Operating System
 Windows XP
 Monitor
 17" SVGA

 
  Data Capture and Display  
 
 Maximum Speed
 6 ratiometric points per second
 100 single-wavelength points per second
 Time Lapse
 Up to 1 measurement every 10 minutes
 Field of View
 Rectangular area, user-definable
 Graphic Display
 Real-time during experiment
 Data Storage
 Format
 Tab-delimited ASCII

 
                          OTHER
 
 Calibration
 From standard solutions, formula, or
 in situ
 Ions
 Works with hundreds of fluorescent dyes
 for measuring: Ca2+, pH, Na+, K+,
 Mg+2, H2O2, cyclic ADP Ribose, etc.