InCyt Basic IM¢â

 

  
 

                                              

                                                  (click image to enlarge and get detailed specs)

  Complete Turnkey System

  The InCyt Basic Im¢â Imaging System includes everything needed for calcium and other single excitation and
  ratiometric fluorophore experiments.
  The system includes: an inverted epi-fluorescence/phase contrast microscope, low-light level integrating CCD
  camera, computer controlled filter changer, Mercury-Xenon lamp, image processing computer and data
  acquisition/analysis software.




  Ease-of-use

  The InCyt Basic Im¢â Imaging System was designed in a biomedical research laboratory by scientists for scientists.
  Menu selection appears in the sequence in which experiments are performed.
  Esoteric options that add complexity and are rarely used have been eliminated from the menus.
  The software follows standard Windows graphical-user-interface-protocol.
  The hardware has been tested for reliability and ruggedness, as well as, simplicity of set up and operation.
  New users can learn to use the system and be doing their first experiment in a few hours.





  Data Collection, Analysis & Presentation

  Data can be measured continuously on as many as 50 pre-selected regions of interest.
  The results can displayed in real time or images can be saved for post hoc analysis.
  Saved images can be played back as an animated sequence-ideal for analysis of sub-cellular responses,
  intercellular communication and heterogeneity among the individual cells in a population.
  Ion concentration within cells can be converted to color using standard or custom pseudo-color tables.
  Image sequences can be presented as a montage with user-defined annotation for presentation and publication.
  Data generated by InCyt Basic Im¢â are stored in a standard TIFF format and data files are stored as ASCII text.

 
 

                                                      Specifications

                                                                    Camera Module
 
 Camera
 Monochrome integrating CCD
 Excitation
 120 watt Mercury-Xenon arc lamp for both
 UV and visible light excitation.
 Variable intensity
 Filter Changer
 Sutter Wheel Filter Changer
 Image
 Resolution
 640 x 480 pixels; 10-bit Micro-Pix

 
  Image Acquisition and Data Analysis Workstation
 
 CPU
 2.80 Ghz Pentium 4
 RAM
 512 MB
 Hard Drive
 80 GB
 CD ROM
 48x CD R/W
 Operating System
 Windows XP
 Monitor
 17" SVGA

 
  Data Capture and Display  
 
 Maximum Speed
 1.5 ratiometric images per second
 15 single-wavelength images per second
 Object Definition
 Specify up to 50 user-defined regions for
 separate analysis
 Data Collection
 Options
 (1) Graph ion concentration in each cell
 during experiment.
 (2) Save images for later analysis and
 animated playback
 Image Format
 TIFF
 Data Storage
 Format
 Tab-delimited ASCII

 
                          OTHER
 
 Calibration
 From standard solutions or formula
 10 single-wavelength images per second
 Ions
 Works with hundreds of fluorescent dyes
 for measuring: Ca2+, pH, Na+, K+,
 Mg+2, H2O2, cyclic ADP Ribose, etc.

 
 

 
 
 

 
 

Data Presentation
 
  • Animated playback of images
  • Montages of selected images with user-defined annotation
  • Graphs of ion kinetics in user-defined regions